p450 - publications

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1. J Int Soc Sports Nutr. 2012 Mar 15;9(1):7. [Epub ahead of print]

The influence of a CYP1A2 polymorphism on the ergogenic effects of caffeine.

Womack CJ, Saunders MJ, Bechtel MK, Bolton DJ, Martin M, Luden ND, Dunham W,
Hancock M.

ABSTRACT: BACKGROUND: Although caffeine supplementation improves performance, the
ergogenic effect is variable. The cause(s) of this variability are unknown. A
(C/A) single nucleotide polymorphism at intron 1 of the cytochrome P450 (CYP1A2)
gene influences caffeine metabolism and clinical outcomes from caffeine
ingestion. The purpose of this study was to determine if this polymorphism
influences the ergogenic effect of caffeine supplementation. METHODS: Thirty-five
trained male cyclists (age = 25.0 +/- 7.3 yrs, height = 178.2 +/- 8.8 cm, weight
= 74.3 +/- 8.8 kg, VO2max = 59.35 +/- 9.72 ml . kg-1 . min-1) participated in two
computer-simulated 40-kilometer time trials on a cycle ergometer. Each test was
performed one hour following ingestion of 6 mg . kg-1 of anhydrous caffeine or a
placebo administered in double-blind fashion. DNA was obtained from whole blood
samples and genotyped using restriction fragment length polymorphism-polymerase
chain reaction. Participants were classified as AA homozygotes (N = 16) or C
allele carriers (N = 19). The effects of treatment (caffeine, placebo) and the
treatment x genotype interaction were assessed using Repeated Measures Analysis
of Variance. RESULTS: Caffeine supplementation reduced 40 kilometer time by a
greater (p < 0.05) magnitude in AA homozygotes (4.9%; caffeine = 72.4 +/- 4.2
min, placebo = 76.1 +/- 5.8 min) as compared to C allele carriers (1.8%; caffeine
= 70.9 +/- 4.3 min, placebo = 72.2 +/- 4.2 min). CONCLUSIONS: Results suggest
that individuals homozygous for the A allele of this polymorphism may have a
larger ergogenic effect following caffeine ingestion.

PMID: 22420682 [PubMed - as supplied by publisher]