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The difference between Schisandra chinensis and the vinegar processed one on activity and mRNA expression of CYP1A2, CYP2E1 and CYP3A4 enzymes in rats.


J Ethnopharmacol. 2013 Jan 29;


Authors: Su T, Mao C, Yin F, Yu Z, Lin Y, Song Y, Lu T


Abstract

ETHNOPHARMACOLOGICAL RELEVANCE: Schisandra chinensis was a well-known traditional Chinese herbal medicine, which has been used in clinical for thousands of years. However, the difference between Schisandra chinensis before and after vinegar processing on cytochrome P450 activities were poorly understood. AIM OF THE STUDY: To evaluate the difference of Schisandra chinensis and the vinegar processed one on metabolism of model CYP1A2, CYP2E1 and CYP3A4 probe substrates in rats using a cocktail method based on a developed and validated HPLC method. And the influence on the levels of CYP mRNA was also studied. MATERIALS AND METHODS: Three probe substrates (theophylline, dapsone and chlorozoxazone) were delivered simultaneously to rats by single and multiple doses treatment of the extracts of Schisandra chinensis and the vinegar processed one. The plasma concentration of the three probes were profiled by HPLC and their corresponding pharmacokinetic parameters were calculated. Real-time RT-PCR was performed to determine the effects of chisandra chinensis and the vinegar processed one on the mRNA expression of CYP1A2, CYP2E1 and CYP3A4 in the rat liver. RESULTS: After single and multiple doses treatment, both extracts of Schisandra chinensis and the vinegar processed one can induce CYP3A4 enzyme activity and inhibit CYP1A2 enzyme activity in rats. Furthermore, the inhibitory effect of Schisandra chinensis after vinegar processed was more potent than without vinegar processed one. CYP2E1 enzyme activity could be induced after single dose treatment but inhibited when multiple doses treatment. Moreover, in the mRNA expression levels, the results were in consistent with the pharmacokinetic results. CONCLUSIONS: These results provided useful scientific data for the safe clinical application of Schisandra chinensis and the vinegar processed one combined with other drugs, which can avoid the side effects induced by herb-drug interactions.

PMID: 23376044 [PubMed - as supplied by publisher]