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1. Environ Toxicol Pharmacol. 2012 Feb 8;34(1):23-33. [Epub ahead of print]

Genetic polymorphisms of the CYP3A4, CYP3A5, CYP3A7 and CYP1A2 among the
Jordanian population.

Salameh G, Hadidi KA, Khateeb ME.

Pathology, Microbiology and Forensic Department, University of Jordan, PO Box
293, Amman 11623, Jordan.

Cytochromes P450 (CYP450) plays an extremely vital role in oxidation, reduction,
and peroxidation of numerous endogenous and exogenous compounds, like drugs and
procarcinogens. Mainly, expression occurs in the liver, in varying polymorphic
forms. Therefore, proposed as biomarkers of susceptibility to carcinogenicity and
toxicity. The objective of this study was to find the allelic frequencies of
CYP3A5*2,*3,*4,*5,*6,*7, CYP3A4*1B, CYP3A7*1C and CYP1A2*1C, *1D, *1E, *1F
enzymes in Jordanians, and to compare them with other ethnic groups. We used
polymerase chain reaction-restriction fragment length (PCR-RFLP) to genotype
alleles, and we calculated frequencies using Hardy Weinberg's equation (HWE).
Allelic frequencies results were: CYP3A5*2 (0.2%), CYP3A5*3 (86.6%), CYP3A5*6
(1.7%), CYP*3A5*4,*5*7 not detected, CYP3A4*1B (11.7%), CYP3A7*1C (1.7%). Finally
6.5%, 18.2%, 6.0%, 67.3% were the results of CYP1A2*1C, 1D, 1E and 1F,
respectively. In conclusion, genotyping method and results of this study can be
adopted or used in pharmacotherapy, toxicity and carcinogenic studies in Jordan.

Copyright © 2012 Elsevier B.V. All rights reserved.

PMID: 22426036 [PubMed - as supplied by publisher]