p450 - publications
Evaluation of EROD and CYP3A4 activities in earthworm Eisenia fetida as biomarkers for soil heavy metal contamination.
J Hazard Mater. 2012 Oct 13;
Authors: Cao X, Song Y, Kai J, Yang X, Ji P
In this study, the effects of heavy metals (Cd, Cu, Pb, Zn) on EROD and CYP3A4 activities in the earthworm Eisenia fetida were evaluated to find out their possible induction and potential as biomarkers for soil heavy metal contamination. The earthworms were exposed to increasing concentrations of Cd (0.1-8mgL(-1)), Cu (10-200mgL(-1)), Pb (20-400mgL(-1)) or Zn (50-400mgL(-1)) in filter papers for 48h. EROD activity was significantly changed in dose-dependent manners after exposure to each of the four metals. CYP3A4 activity was significantly induced by Cd and Pb, rather by Cu and Zn. This is the ﬁrst report on heavy metal-induced changes of CYP3A4 activity in earthworms. Among the four heavy metals, Cd was the most potent inducing EROD and CYP3A4. While EROD and CYP3A4 activities showed a similar trend, EROD is more sensitive than CYP3A4 activity in E. fetida as a biomarker for heavy metals pollution.
PMID: 23127277 [PubMed - as supplied by publisher]